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Overview of the protocol steps. The complete directed differentiation process lasts for 37 days, including 28 days of culture in Matrigel. Two days after seeding the hPSCs, the first step involves adding Activin A for 3 days to generate definitive endoderm formation, followed by the addition of <t>FGF4</t> and CHIRON for 4 more days to induce spheroid formation. The resulting spheroids are then embedded in Matrigel and cultured for 28 days, with a passage at day 14 to renew the Matrigel.
Fgf4, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human recombinant fgf4  (MedChemExpress)
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Overview of the protocol steps. The complete directed differentiation process lasts for 37 days, including 28 days of culture in Matrigel. Two days after seeding the hPSCs, the first step involves adding Activin A for 3 days to generate definitive endoderm formation, followed by the addition of <t>FGF4</t> and CHIRON for 4 more days to induce spheroid formation. The resulting spheroids are then embedded in Matrigel and cultured for 28 days, with a passage at day 14 to renew the Matrigel.
Human Recombinant Fgf4, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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Overview of the protocol steps. The complete directed differentiation process lasts for 37 days, including 28 days of culture in Matrigel. Two days after seeding the hPSCs, the first step involves adding Activin A for 3 days to generate definitive endoderm formation, followed by the addition of <t>FGF4</t> and CHIRON for 4 more days to induce spheroid formation. The resulting spheroids are then embedded in Matrigel and cultured for 28 days, with a passage at day 14 to renew the Matrigel.
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fgf4 protein-based growth factor therapeutics - by Bioz Stars, 2026-03
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goat anti-fgf4  (Santa Cruz Biotechnology)
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Overview of the protocol steps. The complete directed differentiation process lasts for 37 days, including 28 days of culture in Matrigel. Two days after seeding the hPSCs, the first step involves adding Activin A for 3 days to generate definitive endoderm formation, followed by the addition of <t>FGF4</t> and CHIRON for 4 more days to induce spheroid formation. The resulting spheroids are then embedded in Matrigel and cultured for 28 days, with a passage at day 14 to renew the Matrigel.
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https://www.bioz.com/result/goat anti-fgf4/product/Santa Cruz Biotechnology
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Overview of the protocol steps. The complete directed differentiation process lasts for 37 days, including 28 days of culture in Matrigel. Two days after seeding the hPSCs, the first step involves adding Activin A for 3 days to generate definitive endoderm formation, followed by the addition of <t>FGF4</t> and CHIRON for 4 more days to induce spheroid formation. The resulting spheroids are then embedded in Matrigel and cultured for 28 days, with a passage at day 14 to renew the Matrigel.
Fibroblast Growth Factor 4 Fgf4, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fibroblast growth factor 4 fgf4/product/PeproTech
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fibroblast growth factor 4 fgf4 - by Bioz Stars, 2026-03
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fgf4  (Sino Biological)
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Sino Biological fgf4
(A) Representative gene expression of Epi lineage, TE lineage, and PrE lineage in E4.5 embryo cells, BPSC (derived from EPSC) after 1 or 2 days of spontaneous differentiation, and EPSC after 2 days of spontaneous differentiation. (B) Flow cytometry was used to sort single positive and double positive cells and exclude PDFFRA positive cells. (C) Immunofluorescence staining of Oct4+/Cdx2+ and Oct4+/Cdx2-cells after 2 days of spontaneous differentiation. Staining for OCT4, CDX2 and GATA6. Scale bar, 20 μm. (D) Immunofluorescence staining of differentiation cells with or without <t>FGF4</t> & Heparin. Staining for OCT4, CDX2 and GATA6. Scale bar, 20 μm. (E) Stacked bar plot showing the percentages of Oct4+/Cdx2+ and Oct4+/Cdx2-cell differentiation with or without FGF4 & Heparin.
Fgf4, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fgf4 growth factor  (PeproTech)
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(A) Representative gene expression of Epi lineage, TE lineage, and PrE lineage in E4.5 embryo cells, BPSC (derived from EPSC) after 1 or 2 days of spontaneous differentiation, and EPSC after 2 days of spontaneous differentiation. (B) Flow cytometry was used to sort single positive and double positive cells and exclude PDFFRA positive cells. (C) Immunofluorescence staining of Oct4+/Cdx2+ and Oct4+/Cdx2-cells after 2 days of spontaneous differentiation. Staining for OCT4, CDX2 and GATA6. Scale bar, 20 μm. (D) Immunofluorescence staining of differentiation cells with or without <t>FGF4</t> & Heparin. Staining for OCT4, CDX2 and GATA6. Scale bar, 20 μm. (E) Stacked bar plot showing the percentages of Oct4+/Cdx2+ and Oct4+/Cdx2-cell differentiation with or without FGF4 & Heparin.
Fgf4 Growth Factor, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant human fgf4 100-31-25  (PeproTech)
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PeproTech recombinant human fgf4 100-31-25
(A) Representative gene expression of Epi lineage, TE lineage, and PrE lineage in E4.5 embryo cells, BPSC (derived from EPSC) after 1 or 2 days of spontaneous differentiation, and EPSC after 2 days of spontaneous differentiation. (B) Flow cytometry was used to sort single positive and double positive cells and exclude PDFFRA positive cells. (C) Immunofluorescence staining of Oct4+/Cdx2+ and Oct4+/Cdx2-cells after 2 days of spontaneous differentiation. Staining for OCT4, CDX2 and GATA6. Scale bar, 20 μm. (D) Immunofluorescence staining of differentiation cells with or without <t>FGF4</t> & Heparin. Staining for OCT4, CDX2 and GATA6. Scale bar, 20 μm. (E) Stacked bar plot showing the percentages of Oct4+/Cdx2+ and Oct4+/Cdx2-cell differentiation with or without FGF4 & Heparin.
Recombinant Human Fgf4 100 31 25, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant human fgf4 100-31-25/product/PeproTech
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Overview of the protocol steps. The complete directed differentiation process lasts for 37 days, including 28 days of culture in Matrigel. Two days after seeding the hPSCs, the first step involves adding Activin A for 3 days to generate definitive endoderm formation, followed by the addition of FGF4 and CHIRON for 4 more days to induce spheroid formation. The resulting spheroids are then embedded in Matrigel and cultured for 28 days, with a passage at day 14 to renew the Matrigel.

Journal: Biology of the Cell

Article Title: Generation of Intestinal and Colonic Organoids Derived From Human Pluripotent Stem Cells

doi: 10.1111/boc.70044

Figure Lengend Snippet: Overview of the protocol steps. The complete directed differentiation process lasts for 37 days, including 28 days of culture in Matrigel. Two days after seeding the hPSCs, the first step involves adding Activin A for 3 days to generate definitive endoderm formation, followed by the addition of FGF4 and CHIRON for 4 more days to induce spheroid formation. The resulting spheroids are then embedded in Matrigel and cultured for 28 days, with a passage at day 14 to renew the Matrigel.

Article Snippet: FGF4 (Human recombinant fibroblast growth factor 4 premium grade, Miltenyi Biotec, cat. no. 130‐109‐391) ▲CRITICAL .

Techniques: Cell Culture

Characterization of definitive endoderm induction and spheroids generation. (a) Immunofluorescence images for FOXA2 and SOX17 of definitive endoderm after 3 days of Activin A induction. (b) Immunofluorescence images of hindgut spheroids following 4 days of FGF4 and Chiron induction, characterized by CDX2 (intestinal marker) and E‐cadherin (epithelial marker) expression. Scale bars = 100 µm.

Journal: Biology of the Cell

Article Title: Generation of Intestinal and Colonic Organoids Derived From Human Pluripotent Stem Cells

doi: 10.1111/boc.70044

Figure Lengend Snippet: Characterization of definitive endoderm induction and spheroids generation. (a) Immunofluorescence images for FOXA2 and SOX17 of definitive endoderm after 3 days of Activin A induction. (b) Immunofluorescence images of hindgut spheroids following 4 days of FGF4 and Chiron induction, characterized by CDX2 (intestinal marker) and E‐cadherin (epithelial marker) expression. Scale bars = 100 µm.

Article Snippet: FGF4 (Human recombinant fibroblast growth factor 4 premium grade, Miltenyi Biotec, cat. no. 130‐109‐391) ▲CRITICAL .

Techniques: Immunofluorescence, Marker, Expressing

(A) Representative gene expression of Epi lineage, TE lineage, and PrE lineage in E4.5 embryo cells, BPSC (derived from EPSC) after 1 or 2 days of spontaneous differentiation, and EPSC after 2 days of spontaneous differentiation. (B) Flow cytometry was used to sort single positive and double positive cells and exclude PDFFRA positive cells. (C) Immunofluorescence staining of Oct4+/Cdx2+ and Oct4+/Cdx2-cells after 2 days of spontaneous differentiation. Staining for OCT4, CDX2 and GATA6. Scale bar, 20 μm. (D) Immunofluorescence staining of differentiation cells with or without FGF4 & Heparin. Staining for OCT4, CDX2 and GATA6. Scale bar, 20 μm. (E) Stacked bar plot showing the percentages of Oct4+/Cdx2+ and Oct4+/Cdx2-cell differentiation with or without FGF4 & Heparin.

Journal: bioRxiv

Article Title: Modeling Post-Gastrula Development via Bidirectional Pluripotent Stem Cells

doi: 10.1101/2025.06.28.662107

Figure Lengend Snippet: (A) Representative gene expression of Epi lineage, TE lineage, and PrE lineage in E4.5 embryo cells, BPSC (derived from EPSC) after 1 or 2 days of spontaneous differentiation, and EPSC after 2 days of spontaneous differentiation. (B) Flow cytometry was used to sort single positive and double positive cells and exclude PDFFRA positive cells. (C) Immunofluorescence staining of Oct4+/Cdx2+ and Oct4+/Cdx2-cells after 2 days of spontaneous differentiation. Staining for OCT4, CDX2 and GATA6. Scale bar, 20 μm. (D) Immunofluorescence staining of differentiation cells with or without FGF4 & Heparin. Staining for OCT4, CDX2 and GATA6. Scale bar, 20 μm. (E) Stacked bar plot showing the percentages of Oct4+/Cdx2+ and Oct4+/Cdx2-cell differentiation with or without FGF4 & Heparin.

Article Snippet: TSM was based on TSM basal medium supplemented with 25 ng/mL FGF4 (Sino Biological 16043-HNAE-100) and 1μg/mL Heparin (Sigma-Aldrich H3149-500KU).

Techniques: Gene Expression, Derivative Assay, Flow Cytometry, Immunofluorescence, Staining, Cell Differentiation